Robert Hausinger

  • structures, x-ray, ligands, membranes, metals, spectroscopy, kinetics

Department of Microbiology and Molecular Genetics
Department of Biochemistry and Molecular Biology

BPS 6193 | web site | hausinge@msu.edu

  • Characterization of metalloenzymes
  • Function of nickel enzymes lactate racemase and urease
  • Function of iron and 2-oxoglutarate dependent hydroxylases

Google Scholar

Recent Publications

New metal cofactors and recent metallocofactor insights.
Hausinger RP.
Curr Opin Struct Biol (2019) Jan 31; 59:1-8.

A structural perspective on the PP-loop ATP pyrophosphatase family.
Fellner M, Hausinger RP, Hu J.
Crit Rev Biochem Mol Biol (2018) Dec; 53(6):607-622.

Nickel-pincer nucleotide cofactor.
Hausinger RP, Desguin B, Fellner M, Rankin JA, Hu J.
Curr Opin Chem Biol (2018) Dec; 47:18-23.

Thermodynamics of Iron(II) and Substrate Binding to the Ethylene-Forming Enzyme.
Li M, Martinez S, Hausinger RP, Emerson JP.
Biochemistry (2018) Oct 2; 57(39):5696-5705.

Analysis of the Active Site Cysteine Residue of the Sacrificial Sulfur Insertase LarE from Lactobacillus plantarum.
Fellner M, Rankin JA, Desguin B, Hu J, Hausinger RP.
Biochemistry (2018) Sep 25; 57(38):5513-5523.

Biosynthesis of the nickel-pincer nucleotide cofactor of lactate racemase requires a CTP-dependent cyclometallase.
Desguin B, Fellner M, Riant O, Hu J, Hausinger RP, Hols P, Soumillion P.
J Biol Chem (2018) Aug 10; 293(32):12303-12317.

The Irving-Williams series and the 2-His-1-carboxylate facial triad: a thermodynamic study of Mn2+, Fe2+, and Co2+ binding to taurine/α-ketoglutarate dioxygenase (TauD).
Li M, Henderson KL, Martinez S, Hausinger RP, Emerson JP.
J Biol Inorg Chem (2018) Jul; 23(5):785-793.

Amazing Diversity in Biochemical Roles of Fe(II)/2-Oxoglutarate Oxygenases.
Herr CQ, Hausinger RP.
Trends Biochem Sci (2018) Jul; 43(7):517-532.

Lactate Racemase Nickel-Pincer Cofactor Operates by a Proton-Coupled Hydride Transfer Mechanism.
Rankin JA, Mauban RC, Fellner M, Desguin B, McCracken J, Hu J, Varganov SA, Hausinger RP.
Biochemistry (2018) Jun 12; 57(23):3244-3251.

A Structural Model of the Urease Activation Complex Derived from Ion Mobility-Mass Spectrometry and Integrative Modeling.
Eschweiler JD, Farrugia MA, Dixit SM, Hausinger RP, Ruotolo BT.
Structure (2018) Apr 3; 26(4):599-606.e3.

Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells.
Müller TA, Struble SL, Meek K, Hausinger RP.
Biochem Biophys Res Commun (2018) Jan 1; 495(1):98-103.

Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist.
Martinez S, Fellner M, Herr CQ, Ritchie A, Hu J, Hausinger RP.
J Am Chem Soc (2017) Aug 30; 139(34):11980-11988.

Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE.
Fellner M, Desguin B, Hausinger RP, Hu J.
Proc Natl Acad Sci U S A (2017) Aug 22; 114(34):9074-9079.

Unexpected complexity in the lactate racemization system of lactic acid bacteria.
Desguin B, Soumillion P, Hausinger RP, Hols P.
FEMS Microbiol Rev (2017) Aug 1; 41(Supp_1):S71-S83.

Correction to Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2.
Martinez S, Hausinger RP.
Biochemistry (2017) Jun 20; 56(24):3158.

Non-thiolate ligation of nickel by nucleotide-free UreG of Klebsiella aerogenes.
Martin-Diaconescu V, Joseph CA, Boer JL, Mulrooney SB, Hausinger RP, Maroney MJ.
J Biol Inorg Chem (2017) Jun; 22(4):497-503.

Global stability of an α-ketoglutarate-dependent dioxygenase (TauD) and its related complexes.
Henderson KL, Li M, Martinez S, Lewis EA, Hausinger RP, Emerson JP.
Biochim Biophys Acta Gen Subj (2017) May; 1861(5 Pt A):987-994.

Biochemical Characterization of AP Lyase and m6A Demethylase Activities of Human AlkB Homologue 1 (ALKBH1).
Müller TA, Tobar MA, Perian MN, Hausinger RP.
Biochemistry (2017) Apr 4; 56(13):1899-1910.

Spectroscopic analyses of 2-oxoglutarate-dependent oxygenases: TauD as a case study.
Proshlyakov DA, McCracken J, Hausinger RP.
J Biol Inorg Chem (2017) Apr; 22(2-3):367-379.

Increased heterocyst frequency by patN disruption in Anabaena leads to enhanced photobiological hydrogen production at high light intensity and high cell density.
Masukawa H, Sakurai H, Hausinger RP, Inoue K.
Appl Microbiol Biotechnol (2017) Mar; 101(5):2177-2188.

ALKBH7 Variant Related to Prostate Cancer Exhibits Altered Substrate Binding.
Walker AR, Silvestrov P, Müller TA, Podolsky RH, Dyson G, Hausinger RP, Cisneros GA.
PLoS Comput Biol (2017) Feb; 13(2):e1005345.