Cheryl Kerfeld

  • structures, virus, cell

MSU-DOE Plant Reseach Laboratory
Department of Biochemistry & Molecular Biology
Lawrence Berkeley National Lab

Plant Biology 222C | web site | kerfeldc@msu.edu

  • Carboxysomes and microcompartments
  • Carbon fixation
  • Cyanobacterial photoprotection

Google Scholar

Recent Publications

Engineering the orange carotenoid protein for applications in synthetic biology.
Dominguez-Martin MA, Kerfeld CA.
Curr Opin Struct Biol (2019) Aug; 57:110-117.

A designed bacterial microcompartment shell with tunable composition and precision cargo loading.
Ferlez B, Sutter M, Kerfeld CA.
Metab Eng (2019) Jul; 54:286-291.

X-ray radiolytic labeling reveals the molecular basis of orange carotenoid protein photoprotection and its interactions with fluorescence recovery protein.
Gupta S, Sutter M, Remesh SG, Dominguez-Martin MA, Bao H, Feng XA, Chan LG, Petzold CJ, Kerfeld CA, Ralston CY.
J Biol Chem (2019) May 31; 294(22):8848-8860.

The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.
Greber BJ, Sutter M, Kerfeld CA.
Structure (2019) May 7; 27(5):749-763.e4.

Structural and spectroscopic characterization of HCP2.
Dominguez-Martin MA, Polívka T, Sutter M, Ferlez B, Lechno-Yossef S, Montgomery BL, Kerfeld CA.
Biochim Biophys Acta Bioenerg (2019) May 1; 1860(5):414-424.

Structural Characterization of a Synthetic Tandem-Domain Bacterial Microcompartment Shell Protein Capable of Forming Icosahedral Shell Assemblies.
Sutter M, McGuire S, Ferlez B, Kerfeld CA.
ACS Synth Biol (2019) Apr 19; 8(4):668-674.

Glycyl Radical Enzyme-Associated Microcompartments: Redox-Replete Bacterial Organelles.
Ferlez B, Sutter M, Kerfeld CA.
MBio (2019) Jan 8; 10:(1).

Heterohexamers Formed by CcmK3 and CcmK4 Increase the Complexity of Beta Carboxysome Shells.
Sommer M, Sutter M, Gupta S, Kirst H, Turmo A, Lechno-Yossef S, Burton RL, Saechao C, Sloan NB, Cheng X, Chan LG, Petzold CJ, Fuentes-Cabrera M, Ralston CY, Kerfeld CA.
Plant Physiol (2019) Jan; 179(1):156-167.

In Vitro Assembly of Diverse Bacterial Microcompartment Shell Architectures.
Hagen AR, Plegaria JS, Sloan N, Ferlez B, Aussignargues C, Burton R, Kerfeld CA.
Nano Lett (2018) Nov 14; 18(11):7030-7037.

Free-electron laser data for multiple-particle fluctuation scattering analysis.
Pande K, Donatelli JJ, Malmerberg E, Foucar L, Poon BK, Sutter M, Botha S, Basu S, Bruce Doak R, Dörner K, Epp SW, Englert L, Fromme R, Hartmann E, Hartmann R, Hauser G, Hattne J, Hosseinizadeh A, Kassemeyer S, Lomb L, Montero SFC, Menzel A, Rolles D, Rudenko A, Seibert MM, Sierra RG, Schwander P, Ourmazd A, Fromme P, Sauter NK, Bogan M, Bozek J, Bostedt C, Schlichting I, Kerfeld CA, Zwart PH.
Sci Data (2018) Oct 2; 5:180201.

Genomes of ubiquitous marine and hypersaline Hydrogenovibrio, Thiomicrorhabdus and Thiomicrospira spp. encode a diversity of mechanisms to sustain chemolithoautotrophy in heterogeneous environments.
Scott KM, Williams J, Porter CMB, Russel S, Harmer TL, Paul JH, Antonen KM, Bridges MK, Camper GJ, Campla CK, Casella LG, Chase E, Conrad JW, Cruz MC, Dunlap DS, Duran L, Fahsbender EM, Goldsmith DB, Keeley RF, Kondoff MR, Kussy BI, Lane MK, Lawler S, Leigh BA, Lewis C, Lostal LM, Marking D, Mancera PA, McClenthan EC, McIntyre EA, Mine JA, Modi S, Moore BD, Morgan WA, Nelson KM, Nguyen KN, Ogburn N, Parrino DG, Pedapudi AD, Pelham RP, Preece AM, Rampersad EA, Richardson JC, Rodgers CM, Schaffer BL, Sheridan NE, Solone MR, Staley ZR, Tabuchi M, Waide RJ, Wanjugi PW, Young S, Clum A, Daum C, Huntemann M, Ivanova N, Kyrpides N, Mikhailova N, Palaniappan K, Pillay M, Reddy TBK, Shapiro N, Stamatis D, Varghese N, Woyke T, Boden R, Freyermuth SK, Kerfeld CA.
Environ Microbiol (2018) Aug; 20(8):2686-2708.

Programmed loading and rapid purification of engineered bacterial microcompartment shells.
Hagen A, Sutter M, Sloan N, Kerfeld CA.
Nat Commun (2018) Jul 23; 9(1):2881.

Light-Driven Chloride Transport Kinetics of Halorhodopsin.
Feroz H, Ferlez B, Lefoulon C, Ren T, Baker CS, Gajewski JP, Lugar DJ, Gaudana SB, Butler PJ, Hühn J, Lamping M, Parak WJ, Hibberd JM, Kerfeld CA, Smirnoff N, Blatt MR, Golbeck JH, Kumar M.
Biophys J (2018) Jul 17; 115(2):353-360.

Structural and functional insights into the unique CBS-CP12 fusion protein family in cyanobacteria.
Hackenberg C, Hakanpää J, Cai F, Antonyuk S, Eigner C, Meissner S, Laitaoja M, Jänis J, Kerfeld CA, Dittmann E, Lamzin VS.
Proc Natl Acad Sci U S A (2018) Jul 3; 115(27):7141-7146.

Engineering nanoreactors using bacterial microcompartment architectures.
Plegaria JS, Kerfeld CA.
Curr Opin Biotechnol (2018) Jun; 51:1-7.

Bacterial microcompartments.
Kerfeld CA, Aussignargues C, Zarzycki J, Cai F, Sutter M.
Nat Rev Microbiol (2018) May; 16(5):277-290.

Fluorescence and Excited-State Conformational Dynamics of the Orange Carotenoid Protein.
Gurchiek JK, Bao H, Domínguez-Martín MA, McGovern SE, Marquardt CE, Roscioli JD, Ghosh S, Kerfeld CA, Beck WF.
J Phys Chem B (2018) Feb 15; 122(6):1792-1800.

Structural and Functional Characterization of a Short-Chain Flavodoxin Associated with a Noncanonical 1,2-Propanediol Utilization Bacterial Microcompartment.
Plegaria JS, Sutter M, Ferlez B, Aussignargues C, Niklas J, Poluektov OG, Fromwiller C, TerAvest M, Utschig LM, Tiede DM, Kerfeld CA.
Biochemistry (2017) Oct 24; 56(42):5679-5690.

Carboxysomes: metabolic modules for CO2 fixation.
Turmo A, Gonzalez-Esquer CR, Kerfeld CA.
FEMS Microbiol Lett (2017) Oct 2; 364:(18).

Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications.
Young EJ, Burton R, Mahalik JP, Sumpter BG, Fuentes-Cabrera M, Kerfeld CA, Ducat DC.
Front Microbiol (2017) 8:1441.

A bioarchitectonic approach to the modular engineering of metabolism.
Kerfeld CA.
Philos Trans R Soc Lond B Biol Sci (2017) Sep 26; 372:(1730).

Raman Optical Activity Reveals Carotenoid Photoactivation Events in the Orange Carotenoid Protein in Solution.
Fujisawa T, Leverenz RL, Nagamine M, Kerfeld CA, Unno M.
J Am Chem Soc (2017) Aug 2; 139(30):10456-10460.

Structure, function and evolution of the cyanobacterial orange carotenoid protein and its homologs.
Kerfeld CA, Melnicki MR, Sutter M, Dominguez-Martin MA.
New Phytol (2017) Aug; 215(3):937-951.

Synthetic OCP heterodimers are photoactive and recapitulate the fusion of two primitive carotenoproteins in the evolution of cyanobacterial photoprotection.
Lechno-Yossef S, Melnicki MR, Bao H, Montgomery BL, Kerfeld CA.
Plant J (2017) Aug; 91(4):646-656.

Additional families of orange carotenoid proteins in the photoprotective system of cyanobacteria.
Bao H, Melnicki MR, Pawlowski EG, Sutter M, Agostoni M, Lechno-Yossef S, Cai F, Montgomery BL, Kerfeld CA.
Nat Plants (2017) Jul 10; 3:17089.

Assembly principles and structure of a 6.5-MDa bacterial microcompartment shell.
Sutter M, Greber B, Aussignargues C, Kerfeld CA.
Science (2017) Jun 23; 356(6344):1293-1297.

β-Carboxysome bioinformatics: identification and evolution of new bacterial microcompartment protein gene classes and core locus constraints.
Sommer M, Cai F, Melnicki M, Kerfeld CA.
J Exp Bot (2017) Jun 1; 68(14):3841-3855.

Structure and functions of Orange Carotenoid Protein homologs in cyanobacteria.
Bao H, Melnicki MR, Kerfeld CA.
Curr Opin Plant Biol (2017) Jun; 37:1-9.

David W. Krogmann, 1931-2016.
Brand JJ, Kerfeld CA, Cramer WA, Govindjee.
Photosynth Res (2017) Apr; 132(1):1-12.

In Vitro Characterization and Concerted Function of Three Core Enzymes of a Glycyl Radical Enzyme - Associated Bacterial Microcompartment.
Zarzycki J, Sutter M, Cortina NS, Erb TJ, Kerfeld CA.
Sci Rep (2017) Feb 16; 7:42757.